After protein purification the next step in the path toward a structure is crystallization. Crystallization requires ultra pure protein. Usually three or more columns are run to obtain high quality protein. A combination of affinity columns, columns that separate based on charge or hydrophobicity, and size exclusion columns are all utilized to produce as pure a protein product as possible. Protein quality can be verified by overloading an SDS Page coomassie stained gel, then looking for only one protein band. While crystallization is a purification step, rarely do mixed protein species generate diffraction quality crystals.
The Structure Biology Core has two TTP LabTech mosquito robots, and a Rigaku Alchemist II, Minstrel system supported with Crystal Trak software. A Gryphon LCP device, is also available through the core. These robots can be used by Structural Biology Core members, or used by non-members on a cost basis.
Rigaku Minstrel & Alchemist Systems Clinical Sciences Research 7705
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Pathology & Immunology