After protein purification the next step in the path toward a structure is crystallization. Crystallization requires ultra pure protein. Usually three or more columns are run to obtain high quality protein. A combination of affinity columns, columns that separate based on charge or hydrophobicity, and size exclusion columns are all utilized to produce as pure a protein product as possible. Protein quality can be verified by overloading an SDS Page coomassie stained gel, then looking for only one protein band. While crystallization is a purification step, rarely do mixed protein species generate diffraction quality crystals.
The Structure Biology Core has two TTP LabTech mosquito robots, and a Rigaku Alchemist II, Minstrel system supported with Crystal Trak software, is also available through the core. These robots can be used by Structural Biology Core Members.
Rigaku Minstrel & Alchemist Systems Clinical Sciences Research Building 7705
Email Contact Rick Stegeman
Pathology & Immunology